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    當前位置:首頁 > 技術文章 > nanolight GLuc GLOW 檢測試劑簡介

    nanolight GLuc GLOW 檢測試劑簡介

    更新時間:2022-06-23      點擊次數:2057



    nanolight GLuc GLOW 檢測試劑簡介


    CAT#320 GLuc GLOW Assay

    CAT#320(b).product-thumb

     

     

     

    推出用于高通量篩選 (HTS) 的新型一步 GLOW 檢測試劑盒。該套件包含一個緩沖液,可將您的 Gaussia 信號延長數小時。Gaussia 在這個緩沖系統中的半衰期在 2 到 3 小時之間。


    GLOW 檢測試劑盒內容

    (足以進行大約 1000 次檢測)
    • 50 ml  NanoFuel® GLOW 高斯熒光素酶試劑

    • 1 ml    50x Coelenterazine Substrate 用于 GLOW 檢測

    優點

    • 一步測定試劑(溶解、稀釋和測定緩沖液在一種溶液中)

    • 穩定的發光信號(2-3 小時半衰期)

    • 分泌型高斯熒光素酶的檢測

    • 檢測細胞內表達的高斯熒光素酶

    • 低背景,因此高信噪比

     

    Nanolight2022年價格表


    貨號品名規格價格品牌
    102pUC18 Ptilosarcus GFP  25ug25ug4720Nanolight
    108Humanized Ptilosarcus GFP in pCMV25ug4720Nanolight
    109Humanized Renilla Mullerei GFP in pCMV25ug4720Nanolight
    103pUC18  Renilla Reniformis GFP, 25ug   430ug/ml 25ug4720Nanolight
    104Humanized Renilla Mullerei GFP in pUC1825ug4720Nanolight
    105Humanized Ptilosarcus GFP in pUC1825ug4720Nanolight
    106Aequorin Photoprotein in pUC19, 25ug25ug4720Nanolight
    107Obelin Photoprotein in pUC19, 25ug25ug4720Nanolight
    120pART7 Ptilosarcus GFP (5 ug dry), Novel pH indicating "pHluorin5ug4720Nanolight
    121pART27 binary vector lac Z' (5 ug dry),..T-DNA for efficient integration into..5ug4720Nanolight
    200Expressing native Gaussia luciferase without the secretion signal, 1 mg/ml  25ug25ug4720Nanolight
    201Expressing native Gaussia luciferase with the N-terminal secretion signal, 1 mg/ml 25 ug25ug4720Nanolight
    202Humanized Gaussia Luciferase with secretion signal, 20ug20ug4720Nanolight
    203Humanized Gaussia Luciferase, promoterless, with secretion signal, 25ug25ug4720Nanolight
    204Native Renilla Mullerei Luciferase, 25ug25ug4720Nanolight
    205Native Pleuromamma Luciferase, 25ug25ug4720Nanolight
    206Humanized Gaussia Luciferase with C-terminal KDEL endoplasmic retention signal for intracellular expression, 25ug25ug4720Nanolight
    207Humanized Gaussia Luciferase, promoterless; with C-terminal KDEL endoplasmic retention signal for
    intracellular expression control, 25ug
    25ug4720Nanolight
    209Humanized Gaussia luciferase without secretion signal. 25ug25ug4720Nanolight
    300-10Coelenteramine, 4-(5-amino-6-benzylpyrazin-2-yl)phenol. 10 mgs10mg3120Nanolight
    300-100Coelenteramine, 4-(5-amino-6-benzylpyrazin-2-yl)phenol. 100 mgs, (2x50mgs)2x50mg14000Nanolight
    300-50Coelenteramine, 4-(5-amino-6-benzylpyrazin-2-yl)phenol. 50 mgs50mg9200Nanolight
    301-1Lyophilized h-Coelenterazine (mono-dehydroxy derivative also called benzyl-CTZ) 99%. 1 mg (2 x500 ug). 2x500ug880Nanolight
    301-10Lyophilized h-Coelenterazine (mono-dehydroxy derivative also called benzyl-CTZ) 99%  10mgs (2 x 5 mgs)2x5mg5680Nanolight
    3011-1Water soluble h-Coelenterazine for in vivo use. 1mg (2x 500 ug), 2x500ug2000Nanolight
    3011-10Water soluble h-Coelenterazine for in vivo use. 10mgs (20x500 ug)20x500ug14320Nanolight
    3011-10CWater soluble h-Coelenterazine for in vivo use. 10mgs (20x500 ug) plus 10 vials of control20x500ug20640Nanolight
    3011-500Water soluble h-Coelenterazine for in vivo use. 500 ug, 500ug1200Nanolight
    3012-1Water soluble h-Coelenterazine. Reconstitution in 10 ml aqueous buffer. Will result in a 50 µM CTZ solution.
    For in vitro use, 1 tube
    1tube640Nanolight
    3012-10Water soluble h-Coelenterazine. Reconstitution in 10 ml aqueous buffer. Will result in a 50 µM CTZ solution.
    For in vitro use. 10 tubes
    10tubes5040Nanolight
    301-5Lyophilized h-Coelenterazine (mono-dehydroxy derivative also called benzyl-CTZ) 99%  5 mgs5mg3440Nanolight
    301-500Lyophilized h-Coelenterazine (mono-dehydroxy derivative also called benzyl-CTZ) 99%. 500 ug500ug480Nanolight
    301-INJ-1  1 kit. For in vivo use. Contains 2 vials of 500ug h-CTZ  99%   and 0.5 ml Fuel-Inject sterile diluent 1kit1520Nanolight
    301-inj-10 10 kits. For in vivo use. Each lit contains 2 vials of 500ug h-CTZ  99%  and 0.5 ml Fuel-Inject sterile diluent 10kits12720Nanolight
    303-1Lyophilized Native Coelenterazine free base 97% pure, 1 mg (2 x500ug)2x500ug720Nanolight
    303-10Lyophilized Native Coelenterazine free base, 97% pure, 10mgs (2x5mgs), 2x5mg5360Nanolight
    3031-1Water soluble coelenterazine for in vivo use. 1mg (2x 500 ug), 2x500ug2000Nanolight
    3031-10Water soluble Coelenterazine for in vivo use. 10mgs (20x500 ug), 20x500ug14320Nanolight
    3031-10CWater soluble Coelenterazine for in vivo use. 10mgs (20x500 ug) plus 10 vials of control20x500ug20640Nanolight
    3031-500Water soluble Coelenterazine for in vivo use. 500 ug, 500ug1200Nanolight
    3032-1Water soluble Coelenterazine. Reconstitution in 10 ml aqueous buffer. Will result in a 50 µM CTZ solution.
    For in vitro use. 1 tube
    1tube560Nanolight
    3032-10Water soluble Coelenterazine. Reconstitution in 10 ml aqueous buffer. Will result in a 50 µM CTZ solution.
    For in vitro use. 10 tubes
    10tubes4720Nanolight
    303-5Lyophilized Native Coelenterazine free base,  97% pure, 5mgs,5mg3120Nanolight
    303-500Lyophilized Native Coelenterazine free base 97% pure, 500ug, 500ug400Nanolight
    303-inj-1 1 kit. For in vivo use.  Contains 2 vials of 500ug CTZ native 99.7% and 500ul Fuel-Inject sterile diluent 1kit1520Nanolight
    303-inj-10 10 kits. For in vivo use. Each kit contains 2 vials of 500ug CTZ native 99.7%  and  500ul Fuel-Inject sterile diluent 10kits12720Nanolight
    305-1Lyophilized Cypridina Luciferin  99% pure.  Identical to Vargula Luciferin. 1 mg ( 2x 500ug). 2x500ug2000Nanolight
    305-10Lyophilized Cypridina Luciferin  99% pure.  Identical to Vargula Luciferin. 10 mg, (2 x 5mgs)2x5mg15600Nanolight
    305-5Lyophilized Cypridina Luciferin  99% pure.  Identical to Vargula Luciferin.   5 mgs5mg9200Nanolight
    305-500Lyophilized Cypridina Luciferin  99% pure.  Identical to Vargula Luciferin.  500ug500ug1200Nanolight
    306-1000Firefly Luciferin (Photinus species)  D-Luciferin free acid, 1000mgs (4 x 250 mgs). 4x250mg5600Nanolight
    306-250Firefly Luciferin (Photinus species)  D-Luciferin free acid, 250 mgs. 250mg1680Nanolight
    306-500  Firefly Luciferin (Photinus species)  D-Luciferin free acid, 500 mgs (2 x 250 mgs). 2x250mg3360Nanolight
    307L-1Aeqorin Photoprotein 95% pure, in liquid form, 1ml (2 x 500ul)2x500ul7920Nanolight
    307L-500Aeqorin Photoprotein 95% pure, in liquid form, 500ul500ul4720Nanolight
    307P-100Lyophilized Aequorin Photoprotein 95%. 100ug100ug2800Nanolight
    309-1000Coenzyme A  Reagent Grade Free Acid. 1 gram packed in 4 x 250 milligrams. 4x250mg8960Nanolight
    309-250Coenzyme A  Reagent Grade Free Acid, 250 milligrams. 250mg2800Nanolight
    309-500Coenzyme A  Reagent Grade Free Acid. 500 milligrams packed in 2 x 250 milligrams. 2x250mg5600Nanolight
    312-1Renilla Mullerei Luciferase (recombinant) 95%  purity, 1 mg 1mg20000Nanolight
    312-100Renilla Mullerei Luciferase (recombinant) 95%  purity, 100ug100ug3600Nanolight
    315-1 Ptilosarcus Green Fluorescent protein.1 mg1mg4720Nanolight
    315-10 Ptilosarcus Green Fluorescent protein.10 mg10mg15920Nanolight
    316-1 R. Reniformis Green Fluorescent protein.  80% pure, 1 mg1mg4720Nanolight
    316-10 R. Reniformis Green Fluorescent protein.  80% pure, 10 mg10mg15920Nanolight
    318-50 NanoFuel Assay For Firefly Luciferase. Includes: 50ml Buffer and 1 vial of Luciferin Substrate1kit4720Nanolight
    318L-50 NanoFuel Assay For Firefly Luciferase with Lysis. Includes: 50ml Buffer, 1 vial of Luciferin Substrate,
    50ml of Lysis Buffer
    1kit5200Nanolight
    319-50 NanoFuel FLASH Assay for Gaussia Luciferase. Includes: 50ml Gaussia Dilution Buffer,
    50ml Coelenterazine Dilution Buffer, 50ml Lysis Buffer, 1ml CTZ substrate
    1kit4400Nanolight
    320-10 NanoFuel GLOW Assay for Gaussia Luciferase. Includes: 10ml reagent, 0.2ml CTZ substrate1kit1200Nanolight
    320-50 NanoFuel GLOW Assay for Gaussia Luciferase. Includes: 50ml reagent, 1ml CTZ substrate1kit4400Nanolight
    321-1Native Gaussia Luciferase 95%. Recombinant protein 1mg.  Comes with 1 x 1.5 ml reconstitution buffer.kit20000Nanolight
    321-100Native Gaussia Luciferase 95%. Recombinant protein, 100ug.  Comes with 1 x 1.5 ml reconstitution buffer.kit3600Nanolight
    324-50NanoFuel FLASH Assay for Oplophorus Luciferases. Includes: 50ml reagent, 1ml substratekit4400Nanolight
    324L-50NanoFuel FLASH Assay for Oplophorus Luciferases. Includes: 50ml reagent, 1ml substrate, 50ml lysis bufferkit4880Nanolight
    325-10 NanoFuel GLOW Assay for Oplophorus Luciferases: Includes: 10ml reagent, 0.2ml substratekit1200Nanolight
    325-50 NanoFuel GLOW Assay for Oplophorus Luciferases: Includes: 50ml reagent, 1ml substratekit4400Nanolight
    333 NanoFuel 5x Universal Lysis Buffer, 50ml50ml1200Nanolight
    340-1Lyophilized CTZ 400a,1-bisdeoxycoelenterazine,DI-dephdrocoelenterazine,"DeepBlueC".  98%,  1mg (2x500ug) 2x500ug1040Nanolight
    340-10Lyophilized CTZ 400a,1-bisdeoxycoelenterazine,DI-dephdrocoelenterazine,"DeepBlueC". 98%, 10mgs (2 x 5 mgs)2x5mg7600Nanolight
    340-5Lyophilized CTZ 400a,1-bisdeoxycoelenterazine,DI-dephdrocoelenterazine,"DeepBlueC". 98%, 5 mgs.5mg4400Nanolight
    340-500Lyophilized CTZ 400a, 1-bisdeoxycoelenterazine,DI-dephdrocoelenterazine,"DeepBlueC". 98%,  500ug.500ug560Nanolight
    345-1Lyophilized Coelenterazine-Flouride or Coelenterazine F. 1 mg(2 x500ug) 2x500ug1520Nanolight
    345-10Lyophilized Coelenterazine-Flouride or Coelenterazine F. 10 mgs (2 x 5mgs)2x5mg11120Nanolight
    345-5Lyophilized Coelenterazine-Flouride or Coelenterazine F. 5 mgs5mg6320Nanolight
    345-500Lyophilized Coelenterazine-Flouride or Coelenterazine F. 500ug. 500ug880Nanolight
    346-1Lyophilized eCTZ-For e Coelenterazine-Flouride 1 mgs (2 x500ug)2x500ug1520Nanolight
    346-10 eCTZ-For e Coelenterazine-Flouride. 10mgs10mg11120Nanolight
    346-500Lyophilized eCTZ-For e Coelenterazine-Flouride 500ug. 500ug880Nanolight
    350-10Coelenteramide, 10 mgs 10mg2400Nanolight
    350-100Coelenteramide, 100mgs (2 x 50 mgs)  2x50mg11600Nanolight
    350-50Coelenteramide, 50 mgs  50mg7600Nanolight
    355-1Lyophilized e Coelenterazine, 1 mg( 2 x500ug). 2x500ug1040Nanolight
    355-10e Coelenterazine 10mgs.10mg7600Nanolight
    355-500Lyophilized e Coelenterazine, 500ug500ug560Nanolight
    355-inj-11 kit.  For in vivo use. Contains 2 vials of 500ug e-CTZ 97%  and 0.5ml Fuel-Inject sterile diliuent  1kit1520Nanolight
    355-inj-1010 kits.  For in vivo use. Each kit contains 2 vials of 500ug e-CTZ 97% and 0.5ml Fuel-Inject sterile diliuent 10kits12720Nanolight
    360 Prolume Purple sampler kit. It contains 250 ug of each. #361 methoxy v-CTZ, #363 methoxy  CTZ-I,
    #365 methoxy eCTZ-f, #367 methoxt CTZ and #369 methoxt eCTZ.
    1kit4720Nanolight
    361-1Lyophilized Methoxy vCTZ Methoxy  1mg (4 x 250ug). 4x250ug2800Nanolight
    361-10Methoxy vCTZ Methoxy  10 mgs10mg21600Nanolight
    361-250Lyophilized Methoxy vCTZ Methoxy  250ug. 250ug1200Nanolight
    363-1Lyophilized Methoxy CTZ-I  1 mgs (4 x 250ug)4x250ug2800Nanolight
    363-10Methoxy CTZ-I 10mgs 10mg21600Nanolight
    363-250Lyophilized methoxy CTZ-I  250ug, 250ug1200Nanolight
    365-1Lyophilized methoxy eCTZ-F, 1mgs (4 x 250ug)4x250ug2800Nanolight
    365-10methoxy eCTZ-F, 10mgs10mg21600Nanolight
    365-250Lyophilized methoxy eCTZ-F, 250ug. 250ug1200Nanolight
    367-1Lyophilized methoxy CTZ methoxy 1mg(4 x250ug). 4x250ug2800Nanolight
    367-10Methoxy CTZ, 10 mgs10mg21600Nanolight
    367-250Lyophilized methoxy CTZ, methoxy ,250ug. 250ug1200Nanolight
    369-1Lyophilized methoxy eCTZ 1mg(2 x 500ug). 2x500ug2800Nanolight
    369-10Lyophilized Methoxy eCTZ,10 mgs (2 x 5mgs)2x5mg21600Nanolight
    3692-1Prolume Purple ( Methoxy eCTZ), water soluble for in vitro use. 10ml/50uM.  1 tube1tube2000Nanolight
    3692-10Prolume Purple ( Methoxy eCTZ), water soluble for in vitro use. 10ml/50uM.  10 tubes.10tubes16800Nanolight
    369-5Lyophilized Methoxy eCTZ,  5 mgs5mg11120Nanolight
    369-500Lyophilized Methoxy eCTZ, 500ug500ug1680Nanolight
    370-1Lyophilized v -Coelenterazine 1mg (2 x500ug). 2x500ug1520Nanolight
    370-10 v -Coeleterazine 10mgs10mg10000Nanolight
    3702-1Water Soluble vCTZ, water soluble for in vitro use. 10ml/50uM.  1 tube1tube1200Nanolight
    3702-10Water Soluble vCTZ, water soluble for in vitro use. 10ml/50uM. 10 tubes 10tubes10160Nanolight
    370-500Lyophilized v -Coeleterazine 500ug. 500ug880Nanolight
    371-50Gaussia(M2) Luciferase Streptavidin, 50ug50ug5200Nanolight
    381-1NanoKaz Luciferase Protein, 1mg1mg23920Nanolight
    381-100NanoKaz Luciferase Protein, 100ug100ug3600Nanolight
    3815-1NanoKaz Luciferase Protein (PBS) 1mg1mg23920Nanolight
    3815-100NanoKaz Luciferase Protein (PBS) 100ug100ug3600Nanolight
    398-1Solvent for V-Coelenterazine and Prolume Purple V (Methoxy-v-Coelenterazine-Methoxy). 1 tube, 1 ml1ml240Nanolight
    398-10Solvent for V-Coelenterazine and Prolume Purple V (Methoxy-v-Coelenterazine-Methoxy). 10  tubes, 1 ml/tube. 10x1ml1920Nanolight
    399-1NanoFuel Solvent, optimized for in vitro use with CTZ native, h-CTZ, CTZ-F and CTZ 400a. 1 tube, 1 ml1ml240Nanolight
    399-10NanoFuel Solvent, optimized for in vitro use with CTZ native, h-CTZ, CTZ-F and CTZ 400a. 1 tube, 10ml/tube. 10x1ml1600Nanolight
    401M Mouse monoclonal Anti-Gluc.  250ul250ul6320Nanolight
    401P Rabbit Polyclonal Anti-Gluc Titer>1:10,000,in 250 ul250ul6320Nanolight
    402PPolyclonal Rabbit Antibody to Ptilosarcus GFP. Titer>1:10,000,in 250 ul, 250ul6320Nanolight
    403PPolyclonal Rabbit Antibody to Renilla Reniformis GFP. Titer>1:10,000,in 250 ul, 250ul6320Nanolight

     

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